Effect of Alcohol Caffeine Concentration on Daphnia Magna

case of inebriant Caffeine Concentration on Daphnia MagnaThe Effect of Alcohol and Caffeine Concentrations on Daphnia MagnaAbstractThe tar come of this prove was to study the effects of alcohol and caffein on the knocker, using the Daphnia Magna as the test subject. The hypothesis was Increasing the slow-wittedness of caffein in which the Daphnia Magna is submerged will ca theatrical role its aggregate rate to increase, while increasing the concentration of alcohol in which it is submerged will cause its heart rate to decrease. To test this hypothesis two Daphnia Magna were open(a) to either alcohol or caffeine and its heart rate recorded by placing it on a microscope slip ones mind and counting the number of heart beats. It was deducted that the alcohol and caffeine had a precise notable effect on the heart, with alcohol slowing it down and caffeine speeding it up. At the highest concentration of alcohol, the heart rate slowed and in turn the higher(prenominal) concentra tion of caffeine imprinted the heart rate by increasing it. I feel that the same concept of the effect of alcohol and caffeine on Daphnia Magna would be the same concept for its effect on humans also sense alcohol is a depressant it tends to slow down the dead clay as Caffeine is well known for giving our bodies a boost of energy.IntroductionIt was obvious to test the effects that alcohol and caffeine take up on the human heart. The effects of alcohol and caffeine were a distinct interest sense they are drugs that are consumed on a large scale in society. However, because it is difficult to perform experiments of this nature on humans and get accurate results, we decided to use Daphnia Magna as a substitute for a human. though humans and Daphnia Magna are different organisms, alcohol and caffeine should have an almost identical effect on them. The effect when both organisms are exposed to the same amounts of alcohol and caffeine should be more noticeable in Daphnia Magna because they are much smaller, so in turn the chemicals will range through the Daphnia Magnas form quicker and effect each cell more.There are many explanations why Daphnia Magna are unremarkably used compared to the use of a human as test subject. Firstly, Daphnia Magna are simple organisms compared to humans thus at that place are less factors that we have no control over what could likely affect the heart rate. Secondly, the heart of the Daphnia Magna is easy to view and examine because of their transparent body form. Thirdly, it is logical to use a Daphnia Magna because the effects of the drug will be observed much more quickly, within moments compared to around half an hour in humans. Also, to get usable results, it would be necessary to supply a human subject with a reasonable large amounts of alcohol or caffeine, which wouldnt be dependable for short term health, as well as unscrupulous. The hypothesis under investigation was developed because depressants, like alcohol tend to de crease the activity of the bodys organs, whilst stimulants like caffeine tend to increase such activity.Methods and MaterialsTwo Daphnia Magna specimen were collected from the habitat container and then placed each in their own hanging drop slide carefully so they would not slide all over like they would a traditional flat slide. This bureau one slide would have one Daphnia Magna testing the varying concentrations of alcohol and the other Caffeine.In caffeine we readied concentrations of 0.25%, 0.05%, 0.75% and 1.0% levels of concentration. One member held a feeding bottle of distilled water to be used in between concentrations. The water allows the Daphnia Magna to rest a moment that way it doesnt die to quickly from the mixed concentrations. One member will begin the experiment by tallying its heart beats per seconds to allow for an average heart rate. The group then subtracted the water from the slide and replaced it with the fist concentration of caffeine counting the heart be at 15 seconds again then quickly taking away the caffeine and replacing it covering fire with distilled water. We repeat this process and collect the data until all concentrations have be used and the data compiled.Alcohol there were 2%, 4%, 6%, 8%, and 10% of concentrations. Using a Microscope as well as a fresh bottle of water to adjust the Daphnia Magna to the stress of the many concentrations makes the experiment a little easier. As we did with the caffeine the group used a fresh Daphnia Magna collected the average heartbeat and began the process of subjecting the Daphnia Magna to the Alcohol concentrations collecting the heart beat data and observing how it changes with each concentration.ResultsResults demonstrates the profound effect the alcohol and caffeine both have on the heart. As shown in figure 1 and 3 the higher the concentration of caffeine the faster the heart becomes. In figures 2 and 4 Alcohol seems to have the opposite affect and slows the heart rate down increas ingly with each concentration. Within our results it fecal matter be determined that when humans consume alcohol that they will experience a slowness and often a more depressed feeling. On the flip side when humans are exposed to caffeine it can be seen that there is a burst of energy Caffeine is a central nervous system stimulant. Your central nervous system includes your brain, spinal cord, and the other nerves in your body. Caffeines main effect on your body is to make you feel more awake and alert for a while (FDA, 2007)Table 1 group data for Caffeine concentrationsCaffeine Concentrations and how they affect the heart rateConcentrationHeart Rate 15 secHeart Rate (BPM)0%532160.25%421680.50%391560.75%281121.0%31124 solve 1 the graph that shows the group average heart rates when exposed to the different concentrations of caffeine provided that the Daphnia Magna heart rate was counted for 15 seconds then calculated to BPMTable 2 Group data for alcohol concentrationsAlcohol concentr ations and how they affect the heart rateConcentrationHeart Rate 15 secHeart Rate (BPM)0%31133.332%271084%24966%12688%197610%1456Figure 2 the graph that shows the group average heart rates when exposed to the different concentrations of alcohol provided that the Daphnia Magna heart rate was counted for 15 seconds then calculated to BPM.Table 3 Class data for caffeine concentrationsWater Control AVG0.25% Caf0.5% Caf0.75% Caf1.0% CafKR216168156112124g145.356849688G2248448452480508Jeep132208164220188G5133.3120112124136Average154.92200193.6206.4208.8Figure 3 the graph that shows the rank average heart rates when exposed to the different concentrations of caffeine. Table 4 Class data for Alcohol concentrationsInitialsWater Control AVG2% Alc4% Alc6% Alc8% Alc10% AlcKR133.3310896687656g154248285236188172G2205.33122401806448Jeep181148152124124108G5205.3240196232212100Average155.8211.2193.8168132.896.8Figure 4 the graph that shows the class average heart rates when exposed to the different concentrations of alcohol.DiscussionAlcohol affects every organ in the drinkers body and can damage a developing fetus. Intoxication can impair brain function and motor skills heavy use can increase risk of authoritative cancers, stroke, and liver disease. (Abuse, 2014)